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Literature summary for 6.5.1.1 extracted from
- Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae (2002), Extremophiles, 6, 469-477.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus shibatae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 100 mM, 85% inhibition, not inhibited below 50 mM | Saccharolobus shibatae |  |
| NaCl | 100 mM, 85% inhibition, not inhibited below 50 mM | Saccharolobus shibatae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.034 | - |
ATP | 65°C, pH 6.5, recombinant DNA-ligase, in the presence of 1mM ATP and 5 mM Mn2+ | Saccharolobus shibatae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | divalent cation required for activity, most effectiv cation together with Mg2+ for native DNA ligase, maximal activity at 5 mM | Saccharolobus shibatae | |
| Mg2+ | divalent cation required for activity, most effectiv cation together with Ca2+ for native DNA ligase, maximal activity at 5 mM | Saccharolobus shibatae | |
| Mn2+ | divalent cation required for activity, most effectiv cation for recombinant DNA ligase, 10times higher efficiency than with Mg2+, maximal activity at 2-5 mM | Saccharolobus shibatae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
gel filtration | Saccharolobus shibatae |
| 67647 | - |
1 * 67647, deduced from nucleotide sequence | Saccharolobus shibatae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | Saccharolobus shibatae | - |
AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus shibatae | Q9P9K9 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purification of recombinant DNA ligase, partially purification of native DNA ligase | Saccharolobus shibatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Saccharolobus shibatae | AMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
| dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | very low activity | Saccharolobus shibatae | dAMP + diphosphate + (deoxyribonucleotide)m+n | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 67647, deduced from nucleotide sequence | Saccharolobus shibatae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | - |
Saccharolobus shibatae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 90 | - |
Saccharolobus shibatae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
15% loss of activity after 5 h | Saccharolobus shibatae |
| 85 | - |
29% loss of activity after 5 h | Saccharolobus shibatae |
| 90 | - |
50% loss of activity after approx. 10 min | Saccharolobus shibatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Saccharolobus shibatae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus shibatae | deduced from nucleotide sequence | - |
5.86 |
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