Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | required for optimal activity of DNA ligase I, but not for DNA ligase II activity | Drosophila melanogaster | |
polyvinyl alcohol | required for optimal activity of DNA ligase I but not for DNA ligase II activity | Drosophila melanogaster |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NEM | - |
Drosophila melanogaster |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
ATP | DNA ligase I | Drosophila melanogaster | |
0.03 | - |
ATP | DNA ligase II | Drosophila melanogaster |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | DNA ligase I | Drosophila melanogaster | 5737 | - |
nucleus | DNA ligase II | Drosophila melanogaster | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | DNA ligase I is active at low salt concentrations, 0-30 mM KCl, DNA ligase II is active at high salt concentrations, 50-100 mM KCl | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
DNA ligase I and II | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Drosophila melanogaster | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase I and II ligate 5'-phosphoryl and 3'-hydroxyl groups in oligo(dT) in the presence of poly(dA). DNA ligase II can join oligo(dT)*poly(rA) | Drosophila melanogaster | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |