Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.5.1.1 extracted from

  • Takahashi, M.; Senshu, M.
    Two distinct DNA ligases from Drosophila melanogaster embryos (1987), FEBS Lett., 213, 345-352.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol required for optimal activity of DNA ligase I, but not for DNA ligase II activity Drosophila melanogaster
polyvinyl alcohol required for optimal activity of DNA ligase I but not for DNA ligase II activity Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
NEM
-
Drosophila melanogaster

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0027
-
ATP DNA ligase I Drosophila melanogaster
0.03
-
ATP DNA ligase II Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm DNA ligase I Drosophila melanogaster 5737
-
nucleus DNA ligase II Drosophila melanogaster 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information DNA ligase I is active at low salt concentrations, 0-30 mM KCl, DNA ligase II is active at high salt concentrations, 50-100 mM KCl Drosophila melanogaster

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
DNA ligase I and II
-

Source Tissue

Source Tissue Comment Organism Textmining
embryo
-
Drosophila melanogaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase I and II ligate 5'-phosphoryl and 3'-hydroxyl groups in oligo(dT) in the presence of poly(dA). DNA ligase II can join oligo(dT)*poly(rA) Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
?