Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Haemophilus influenzae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | 0.0015 | ATP | ATP | Haemophilus influenzae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Mn2+ is more effective than Mg2+ at 0.5-1 mM | Haemophilus influenzae | |
Mg2+ | Mn2+ or Mn2+ required | Haemophilus influenzae | |
Mn2+ | Mg2+ or Mn2+ required | Haemophilus influenzae | |
Mn2+ | Mn2+ is more effective than Mg2+ at 0.5-1 mM | Haemophilus influenzae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haemophilus influenzae | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate | initial step in DNA ligation is the formation of a covalent enzyme-adenylate intermediate | Haemophilus influenzae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | efficient strand joining on a single nicked DNA | Haemophilus influenzae | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |