Literature summary for 6.5.1.1 extracted from

Sekiguchi, J.; Shuman, S.
Ligation of RNA-containing duplex by vaccinia DNA ligase (1997), Biochemistry, 36, 9073-9079.

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Organism

Organism	UniProt	Comment	Textmining
Vaccinia virus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m	repairs nicked duplex DNA substrates of a 5'-phosphate terminated strand and a 3'-hydroxyl-terminated strand annealed to a bridging template strand. Rapidly and efficiently joins a 3'OH RNA to 5'-phosphate DNA when the reacting polynucleotides are annealed to a bridging DNA strand. Ligation of 3'-OH DNA to 5'-phosphate RNA is 0.2% of the rate of RNA- to-DNA ligation. Requirement for B-form helical conformation on the 5'-phosphate side of the nick. Weak activity in RNA-to-RNA ligation on a bridging DNA template, incapable of joining two DNAs anneald on an RNA template	Vaccinia virus	AMP + diphosphate + (deoxyribonucleotide)n+m	-	?

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Release 2023.1 (January 2023)