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Literature summary for 6.5.1.1 extracted from
- Two DNA ligase activities from calf thymus (1973), Biochem. Biophys. Res. Commun., 53, 910-916.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Bos taurus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 85000 | - |
and a higher MW form of MW 175000, gel filtration, sucrose density gradient centrifugation | Bos taurus |
| 175000 | - |
DNA ligase I, gel filtration, sucrose density gradient centrifugation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | converts nicked circular DNA molecules to a covalently closed form | Bos taurus | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
DNA ligase II, rapid loss of activity above | Bos taurus |
| 42 | - |
DNA ligase II, 5 min, 50% inactivation | Bos taurus |
| 50 | - |
DNA ligase I is stable to prolonged heating | Bos taurus |
| 52 | - |
DNA ligase I, 5 min, 50% inactivation | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | 8 | DNA ligase I, in Tris-HCl buffer | Bos taurus |
| 7.8 | - |
DNA ligase II | Bos taurus |
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