Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | Mn2+ and Ca2+ cannot replace Mg2+ in activation | Homo sapiens | |
Mn2+ | Mn2+ cannot replace Mg2+ in activation | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
DNA ligase II, gel filtration | Homo sapiens |
190000 | - |
and a lower MW form of MW 95000, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
EUE cell | heteroploid | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol w/w, 3 mg/ml bovine serum albumin, stable for up to 7 months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | DNA ligase can reconstitute the transforming activity of Bacillus subtilis DNA inactivated by pancreatic DNAse, unable to use a hybrid substrate where an interrupted polydeoxynucleotide is annealed to a polyribonucleotide | Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |