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Literature summary for 6.5.1.1 extracted from
- Purification and properties of a DNA ligase from a soluble DNA replication complex (1980), Biochim. Biophys. Acta, 606, 202-213.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | required for optimal activity | Homo sapiens |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| actinomycin | - |
Homo sapiens | |
| Ca2+ | CaCl2 inhibits Mg2+-catalyzed reaction | Homo sapiens | |
| diphosphate | - |
Homo sapiens | |
| Ethidium bromide | - |
Homo sapiens | |
| Mn2+ | MnCl2 inhibits Mg2+-catalyzed reaction | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
ATP | DNA terminal phosphate | Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | 3fold stimulation at 70 mM KCl, 4fold stimulation at 150 mM KCl | Homo sapiens | |
| Na+ | KCl is required for optimal activity, 3fold stimulation at 30 mM NaCl, 4fold stimulation at 150 mM NaCl | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 105000 | - |
glycerol density gradient sedimentation | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| KB cell | adenovirus type 2-infected | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | - |
Homo sapiens | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 30 | - |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | 20°C: 55% of maximal activity, 37°C: about 70% of maximal activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8.5 | approximately 70% of maximal activity at pH 7.0 and 8.5 | Homo sapiens |
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