Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Polyethylene glycol | polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations | Tequatrovirus T4 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
K+ | extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate | Tequatrovirus T4 | |
Na+ | extent of inhibition varies with the terminal sequence of the duplex DNA used as substrate. Resistance to inhibition in the order from strong to weak: HindIII, PstI, EcoRI, BamHI, SalI in cohesive end ligation, and EcoRV, ScaI, PvuII, NruI in blunt end ligation | Tequatrovirus T4 |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations | Tequatrovirus T4 | |
Na+ | polyethylene glycol 6000, 15%, stimulates, when NaCl and KCl are both absent. With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation are increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intramolecular ligation occurs at low salt concentrations, 0.1 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations | Tequatrovirus T4 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m | With 10% polyethylene glycol 6000, both cohesive end ligation and blunt end ligation is increased at high concentrations of salt, 150-200 mM NaCl, or 200-250 mM KCl. With 10% polyethylene glycol 6000, intermolecular and intamolecular ligation occurs at low salt concentrations, 0.100 mM NaCl or 0-150 mM KCl. Only linear oligomers are formed by intermolecular ligation at the high concentrations | Tequatrovirus T4 | AMP + diphosphate + (deoxyribonucleotide)n+m | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 16 | when polyethylene glycol is not present | Tequatrovirus T4 |
37 | - |
with 10% polyethylene glycol 6000 intermolecular ligation at high concentrations of salt is stimulated by raising the temperature from 19°C to 37°C | Tequatrovirus T4 |