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Literature summary for 6.5.1.1 extracted from

  • Tomkinson, A.E.; Levin, D.S.
    Mammalian DNA ligases (1997), BioEssays, 19, 893-901.
    View publication on PubMed
Search for more literature for 6.5.1.1

Activating Compound

Activating Compound Comment Organism Structure
XRCC1 DNA ligase III-alpha forms a complex with the DNA single-strand break repair protein XRCC1 Mammalia

Cloned(Commentary)

Cloned (Comment) Organism
DNA ligase I, III-alpha, III-beta, IV Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
ATP inhibits the last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation Mammalia
pyridoxal 5'-phosphate inhibits last step of the reaction, which involves cleavage of the diphosphate bond of the DNA-adenylate intermediate and phosphodiester bond formation Mammalia

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Drosophila melanogaster 5634
-
nucleus
-
 Mammalia 5634
-
nucleus
-
 Homo sapiens 5634
-
nucleus
-
 Bos taurus 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 x * 125000, denaturing PAGE Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Drosophila melanogaster
-
 ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Homo sapiens
-
 ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Saccharomyces cerevisiae
-
 ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Bos taurus
-
 ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Vaccinia virus
-
 ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mammalia DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Mammalia DNA ligase I is involved in DNA replication ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-
Mammalia
-
 DNA ligase I, II, III-alpha, III-beta, and IV. DNA ligase II appears to be derived from DNA ligase III by a proteolytic mechanism
-
Saccharomyces cerevisiae
-
-
-
Schizosaccharomyces pombe
-
-
-
Vaccinia virus
-
-
-
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DNA ligase I Bos taurus
DNA ligase I, II, III, and IV Mammalia

Reaction

Reaction Comment Organism Reaction ID
ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate mechanism: 1. formation of a covalent enzyme-adenylate complex, the ATP is cleaved to AMP and diphosphate with adenylyl residue linked by a phosphoramidate bond to the epsilon-amino group of a specific lysine residue at the active site of the protein, 2. the activated AMP residue of the DNA ligase-adenylate intermediate is transferred to the 5'-phosphate terminus of a single strand break in double-stranded DNA to generate a covalent DNA-AMP complex with a 5'-5' phosphoanhydride bond, 3. in the final step of DNA ligation, unadenylated DNA ligase is required for the generation of a phosphodiester bond and catalyzes displacement of the AMP residue through attack by adjacent 3'-hydroxyl group of the adenylylated site Mammalia
a

Source Tissue

Source Tissue Comment Organism Textmining
germ male meiotic cell, ligase III-beta is expressed only in male meiotic germ cells Mammalia
-
testis
-
 Homo sapiens
-
testis
-
 Bos taurus
-
thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Mammalia AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Saccharomyces cerevisiae AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Bos taurus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Xenopus laevis AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Schizosaccharomyces pombe AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Vaccinia virus AMP + diphosphate + (deoxyribonucleotide)n+m
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Drosophila melanogaster ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Homo sapiens ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Saccharomyces cerevisiae ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Bos taurus ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Vaccinia virus ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase III-beta is expressed only in male meiotic germ cells, suggesting a role for this isoform in meiotic recombination Mammalia ?
-
 ?
ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA ligase I is involved in DNA replication Mammalia ?
-
 ?

Subunits

Subunits Comment Organism
?
-
 Drosophila melanogaster
?
-
 Homo sapiens
?
-
 Saccharomyces cerevisiae
?
-
 Schizosaccharomyces pombe
? x * 125000, denaturing PAGE Bos taurus