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Literature summary for 6.3.5.7 extracted from

  • Feng, L.; Sheppard, K.; Tumbula-Hansen, D.; Soell, D.
    Gln-tRNAGln formation from Glu-tRNAGln requires cooperation of an asparaginase and a Glu-tRNAGln kinase (2005), J. Biol. Chem., 280, 8150-8155.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Methanothermobacter thermautotrophicus

Protein Variants

Protein Variants Comment Organism
D178E glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable Methanothermobacter thermautotrophicus
D178N glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable Methanothermobacter thermautotrophicus
K254E glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable Methanothermobacter thermautotrophicus
T101A glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable Methanothermobacter thermautotrophicus
T101S hydrolyzes about 10% of glutamine compared to wild-type enzyme. Compared to wild-type enzyme, the mutant enzyme converts approximately half as much mischarged tRNA substrate to product Methanothermobacter thermautotrophicus
T177S mutant enzyme hydrolyzes the same amount of glutamine as the wild-type enzyme. As the wild-type enzyme, the mutant enzyme transforms most of Glu-tRNAGln to Gln-tRNAGln Methanothermobacter thermautotrophicus
T177V glutamine hydrolysis is negligible. Gln-tRNAGln formation is undetectable Methanothermobacter thermautotrophicus

Organism

Organism UniProt Comment Textmining
Methanothermobacter thermautotrophicus
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Purification (Commentary)

Purification (Comment) Organism
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Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Glu-tRNAGln + L-glutamine GatDE is a heterodimeric amidotransferase. GatD acts as a glutaminase but only in the presence of both Glu-tRNAGln and the other subunit, GatE. The fact that only Glu-tRNAGln but not tRNA Gln could activate the glutaminase activity of GatD suggests that glutamine hydrolysis is coupled tightly to transamidation. GatE is a Glu-tRNAGln kinase that activates Glu-tRNAGln via gamma-phosphorylation Methanothermobacter thermautotrophicus ADP + phosphate + Gln-tRNAGln + L-glutamate
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Synonyms

Synonyms Comment Organism
GluAdT GatDE
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Methanothermobacter thermautotrophicus