Cloned (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Protein Variants | Comment | Organism |
---|---|---|
D178E | glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable | Methanothermobacter thermautotrophicus |
D178N | glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable | Methanothermobacter thermautotrophicus |
K254E | glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable | Methanothermobacter thermautotrophicus |
T101A | glutamine hydrolysis is negligible, Gln-tRNAGln formation is undetectable | Methanothermobacter thermautotrophicus |
T101S | hydrolyzes about 10% of glutamine compared to wild-type enzyme. Compared to wild-type enzyme, the mutant enzyme converts approximately half as much mischarged tRNA substrate to product | Methanothermobacter thermautotrophicus |
T177S | mutant enzyme hydrolyzes the same amount of glutamine as the wild-type enzyme. As the wild-type enzyme, the mutant enzyme transforms most of Glu-tRNAGln to Gln-tRNAGln | Methanothermobacter thermautotrophicus |
T177V | glutamine hydrolysis is negligible. Gln-tRNAGln formation is undetectable | Methanothermobacter thermautotrophicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine | GatDE is a heterodimeric amidotransferase. GatD acts as a glutaminase but only in the presence of both Glu-tRNAGln and the other subunit, GatE. The fact that only Glu-tRNAGln but not tRNA Gln could activate the glutaminase activity of GatD suggests that glutamine hydrolysis is coupled tightly to transamidation. GatE is a Glu-tRNAGln kinase that activates Glu-tRNAGln via gamma-phosphorylation | Methanothermobacter thermautotrophicus | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GluAdT GatDE | - |
Methanothermobacter thermautotrophicus |