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Literature summary for 6.3.5.7 extracted from

  • Jahn, D.; Kim, Y.C.; Ishino, Y.; Chen, M.W.; Soll, D.
    Purification and functional characterization of the Glu-tRNAGln amidotransferase from Chlamydomonas reinhardtii (1990), J. Biol. Chem., 265, 8059-8064.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
6-diazo-5-oxonorleucine blocking of glutamine-dependent reaction, no inhibition of ammonia-dependent reaction Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Chlamydomonas reinhardtii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
2 * 63000, SDS-PAGE Chlamydomonas reinhardtii
120000
-
gel filtration, glycerol density gradient sedimentation Chlamydomonas reinhardtii

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Glu-tRNAGln + Asn
-
Chlamydomonas reinhardtii ADP + phosphate + Gln-tRNAGln + Asp
-
?
ATP + Glu-tRNAGln + L-glutamine
-
Chlamydomonas reinhardtii ADP + phosphate + Gln-tRNAGln + L-glutamate
-
?
ATP + Glu-tRNAGln + NH4Cl
-
Chlamydomonas reinhardtii ADP + phosphate + Gln-tRNAGln + ?
-
?
additional information the enzyme possesses low glutaminase activity Chlamydomonas reinhardtii ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 63000, SDS-PAGE Chlamydomonas reinhardtii