Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation of glutaminase activity by ATP or ATP-gammaS together with Glu-tRNAGln, results either from an allosteric effect due simply to binding of these analogues to the enzyme or from some structural changes that attend ATP or ATP-gammaS hydrolysis | Streptococcus pyogenes |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2'-O-(trinitrophenyl)adenosine 5'-triphosphate | IC50: 2.4 mM | Streptococcus pyogenes | |
3'-O-(trinitrophenyl)adenosine 5'-triphosphate | IC50: 2.4 mM | Streptococcus pyogenes | |
adenosine 5'-[beta,gamma-methylene]triphosphate | IC50: 2.3 mM | Streptococcus pyogenes | |
ADP | IC50: 0.026 mM | Streptococcus pyogenes | |
ATP-gammaS | IC50: 0.19 mM | Streptococcus pyogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
L-glutamyl-tRNAGlu | - |
Streptococcus pyogenes | |
0.0159 | - |
Gln | - |
Streptococcus pyogenes | |
0.117 | - |
ATP | - |
Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine | Streptococcus pyogenes | organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pyogenes | - |
recombinant enzyme expressed in Escherichia coli | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Glu-tRNAGln + L-glutamine | organisms lacking Gln-tRNA synthetase produce Gln-tRNAGln from misacylated Glu-tRNAGln through the transamidation activity of Glu-tRNAGln amidotransferase. The enzyme hydrolyzes Gln to Glu and NH3, using the latter product to transamidate Glu-tRNAGln in concert with ATP hydrolysis | Streptococcus pyogenes | ADP + phosphate + Gln-tRNAGln + L-glutamate | - |
? | |
ATP-gammaS + Glu-tRNAGln + L-glutamine | - |
Streptococcus pyogenes | ? + phosphate + Gln-tRNAGln + L-glutamate | - |
? | |
additional information | in absence of the amido acceptor, Glu-tRNAGln, the enzyme has basal glutaminase activity that is unaffected by ATP | Streptococcus pyogenes | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.51 | - |
Gln | - |
Streptococcus pyogenes | |
0.59 | - |
ATP | - |
Streptococcus pyogenes |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.026 | - |
IC50: 0.026 mM | Streptococcus pyogenes | ADP | |
0.19 | - |
IC50: 0.19 mM | Streptococcus pyogenes | ATP-gammaS | |
2.3 | - |
IC50: 2.3 mM | Streptococcus pyogenes | adenosine 5'-[beta,gamma-methylene]triphosphate | |
2.4 | - |
IC50: 2.4 mM | Streptococcus pyogenes | 2'-O-(trinitrophenyl)adenosine 5'-triphosphate | |
2.4 | - |
IC50: 2.4 mM | Streptococcus pyogenes | 3'-O-(trinitrophenyl)adenosine 5'-triphosphate |