Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of carB mutants lacking a functional large subunit. The carB mutant shows negligible effects on overall arginine levels. The levels of ornithine, citrulline and arginine remain low in the mutant. the ammonium level of carB mutant cells are similar to the wild-type in air, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-glutamine + HCO3- + H2O | Arabidopsis thaliana | - |
2 ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
genes carA and carB encoding the small and large subunit of the CPSase | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
mesophyll | - |
Arabidopsis thaliana | - |
phloem | - |
Arabidopsis thaliana | - |
root | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-glutamine + HCO3- + H2O | - |
Arabidopsis thaliana | 2 ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | small and large subunit, the small subunit contains a class I (trpG)-type glutaminase domain to hydrolyze glutamine to ammonia. carB is composed of three exons encoding a 120 kDa large subunit, consisting of the duplicated synthetase regions and the ATP-binding domains to synthesize carbamoyl phosphate | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
glutamine-hydrolyzing CPSase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | carB expression is partly downregulated by ammonium treatment, while carA expression is unaffected | down |
General Information | Comment | Organism |
---|---|---|
physiological function | carbamoylphosphate synthetase plays a specific physiological role in ammonium assimilation in the mesophyll and phloem for the synthesis and transport of glutamine, glutamate, and derived amino acids, overview | Arabidopsis thaliana |