Literature summary for 6.3.5.5 extracted from

Potel, F.; Valadier, M.H.; Ferrario-Mery, S.; Grandjean, O.; Morin, H.; Gaufichon, L.; Boutet-Mercey, S.; Lothier, J.; Rothstein, S.J.; Hirose, N.; Suzuki, A.
Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana - roles of glutamate synthases and carbamoylphosphate synthetase in leaves (2009), FEBS J., 276, 4061-4076.

Search for more literature for 6.3.5.5

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of carB mutants lacking a functional large subunit. The carB mutant shows negligible effects on overall arginine levels. The levels of ornithine, citrulline and arginine remain low in the mutant. the ammonium level of carB mutant cells are similar to the wild-type in air, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Arabidopsis thaliana	5739	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 ATP + L-glutamine + HCO3- + H2O	Arabidopsis thaliana	-	2 ADP + phosphate + L-glutamate + carbamoyl phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	genes carA and carB encoding the small and large subunit of the CPSase	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	-	Arabidopsis thaliana	-
mesophyll	-	Arabidopsis thaliana	-
phloem	-	Arabidopsis thaliana	-
root	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 ATP + L-glutamine + HCO3- + H2O	-	Arabidopsis thaliana	2 ADP + phosphate + L-glutamate + carbamoyl phosphate	-	?

Subunits

Subunits	Comment	Organism
heterodimer	small and large subunit, the small subunit contains a class I (trpG)-type glutaminase domain to hydrolyze glutamine to ammonia. carB is composed of three exons encoding a 120 kDa large subunit, consisting of the duplicated synthetase regions and the ATP-binding domains to synthesize carbamoyl phosphate	Arabidopsis thaliana

Synonyms

Synonyms	Comment	Organism
glutamine-hydrolyzing CPSase	-	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Arabidopsis thaliana

Expression

Organism	Comment	Expression
Arabidopsis thaliana	carB expression is partly downregulated by ammonium treatment, while carA expression is unaffected	down

General Information

General Information	Comment	Organism
physiological function	carbamoylphosphate synthetase plays a specific physiological role in ammonium assimilation in the mesophyll and phloem for the synthesis and transport of glutamine, glutamate, and derived amino acids, overview	Arabidopsis thaliana

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)