Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | CPSII GATase domain as well as the C-terminal allosteric regulatory domain are essential, critical dependence on the apicomplexan CPSII GATase domain in vivo, overview | Toxoplasma gondii |
Application | Comment | Organism |
---|---|---|
drug development | CPSII is a potential parasite-selective target for drug development | Toxoplasma gondii |
Cloned (Comment) | Organism |
---|---|
construction and expression of a functional minigene of CPSII for functional complementation of mutants, overview | Toxoplasma gondii |
Protein Variants | Comment | Organism |
---|---|---|
C345A | site-directed mutagenesis of an essential catalytic triad residue of the GATase domain, the mutation completely abolishes complementation activity and GATase activity | Toxoplasma gondii |
additional information | disruption of Toxoplasma gondii CPSII induces a severe uracil auxotrophy with no detectable parasite replication in vitro and complete attenuation of virulence in mice. A CPSII cDNA minigene efficiently complements the uracil auxotrophy of CPSII-deficient mutants, restoring parasite growth and virulence, engineered mutations within, or proximal to, but the catalytic triad of the N-terminal glutamine amidotransferase domain inactivates the complementation activity of CPSII and demonstrates a critical dependence on the apicomplexan CPSII GATase domain in vivo | Toxoplasma gondii |
N348A | site-directed mutagenesis, the mutation reduces complementation activity, GATase activity and the initial growth rate, but the N348A mutant exhibits the high virulence phenotype of the parental RH strain in C57Bl/6 mice | Toxoplasma gondii |
N348R | site-directed mutagenesis, the mutation completely abolishes complementation activity and GATase activity | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + HCO3- + H2O | Toxoplasma gondii | allosteric regulation and CPSII regulatory domains, overview | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
strains cps1-1 and RH | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | maintained in human foreskin fibroblasts | Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + HCO3- + H2O | - |
Toxoplasma gondii | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + HCO3- + H2O | allosteric regulation and CPSII regulatory domains, overview | Toxoplasma gondii | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbamoyl phosphate synthetase II | - |
Toxoplasma gondii |
CPSII | - |
Toxoplasma gondii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Toxoplasma gondii |