Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Deinococcus radiophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + HCO3- + H2O | Deinococcus radiophilus | - |
ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + HCO3- + H2O | Deinococcus radiophilus NCIMB 10648 | - |
ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Deinococcus radiophilus | - |
NCIMB 10648 | - |
Deinococcus radiophilus NCIMB 10648 | - |
NCIMB 10648 | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially | Deinococcus radiophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamine + HCO3- + H2O | - |
Deinococcus radiophilus | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + HCO3- + H2O | substrate-channelling, the enzyme is also active with NH3 as nitrogen source, cf. EC 6.3.4.16 | Deinococcus radiophilus | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + HCO3- + H2O | - |
Deinococcus radiophilus NCIMB 10648 | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
ATP + L-glutamine + HCO3- + H2O | substrate-channelling, the enzyme is also active with NH3 as nitrogen source, cf. EC 6.3.4.16 | Deinococcus radiophilus NCIMB 10648 | ADP + phosphate + L-glutamate + carbamoyl phosphate | - |
? | |
additional information | assay method development based on the fixation of 14C from the substrate bicarbonate to give radioactive products. In the coupled assay the carbamoyl phosphate product of the enzyme is converted to carbamoyl aspartate in the presence of aspartate and aspartate transcarbamoylase, substrate-channelling between aspartate aminotransferase and CPSase, overview | Deinococcus radiophilus | ? | - |
? | |
additional information | assay method development based on the fixation of 14C from the substrate bicarbonate to give radioactive products. In the coupled assay the carbamoyl phosphate product of the enzyme is converted to carbamoyl aspartate in the presence of aspartate and aspartate transcarbamoylase, substrate-channelling between aspartate aminotransferase and CPSase, overview | Deinococcus radiophilus NCIMB 10648 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
carbamoyl phosphate synthetase | - |
Deinococcus radiophilus |
CPSase | - |
Deinococcus radiophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Deinococcus radiophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Deinococcus radiophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Deinococcus radiophilus |