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Literature summary for 6.3.5.5 extracted from

  • Kothe, M.; Powers-Lee, S.G.
    Nucleotide recognition in the ATP-grasp protein carbamoyl phosphate synthetase (2004), Protein Sci., 13, 466-475.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A144Q mutant enzyme retains ATP specificity Escherichia coli
D207A mutant enzyme retains ATP specificity Escherichia coli
D207N mutant enzyme retains ATP specificity Escherichia coli
D753A mutant enzyme retains ATP specificity Escherichia coli
D753N mutant enzyme retains ATP specificity Escherichia coli
F755A mutant enzyme retains ATP specificity Escherichia coli
I211S mutant enzyme retains ATP specificity Escherichia coli
P690Q mutant enzyme retains ATP specificity Escherichia coli
S209A mutant enzyme retains ATP specificity Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.004
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant F755A Escherichia coli
0.005
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, wild-type enzyme Escherichia coli
0.006
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207A Escherichia coli
0.007
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753A Escherichia coli
0.008
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753N Escherichia coli
0.009
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207N Escherichia coli
0.009
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant P690Q Escherichia coli
0.015
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D207A Escherichia coli
0.016
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant S209A Escherichia coli
0.02
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D207N Escherichia coli
0.02
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant I211S Escherichia coli
0.021
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q/D207A Escherichia coli
0.029
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, wild-type enzyme Escherichia coli
0.031
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant S209A Escherichia coli
0.034
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q Escherichia coli
0.0383
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant P690Q Escherichia coli
0.064
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D753A Escherichia coli
0.072
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant I211S Escherichia coli
0.121
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D753N Escherichia coli
0.135
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant F755A Escherichia coli
0.154
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q/D207A Escherichia coli
0.285
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli
0.06
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207N Escherichia coli
0.07
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant F755A Escherichia coli
0.09
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant P690Q Escherichia coli
0.09
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, wild-type enzyme Escherichia coli
0.1
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q Escherichia coli
0.11
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D207A Escherichia coli
0.12
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant A144Q/D207A Escherichia coli
0.13
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753N Escherichia coli
0.14
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant D753A Escherichia coli
0.29
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant I211S Escherichia coli
0.35
-
ATP pH 7.6, 25°C, HCO3(-)-dependent ATPase, mutant S209A Escherichia coli
1.51
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D207A Escherichia coli
1.65
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D207N Escherichia coli
1.7
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q/D207A Escherichia coli
1.71
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D753A Escherichia coli
2.43
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant S209A Escherichia coli
3.94
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant A144Q Escherichia coli
4.04
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, wild-type enzyme Escherichia coli
4.77
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant I211S Escherichia coli
5.18
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant F755A Escherichia coli
5.6
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant D753N Escherichia coli
9.01
-
ATP pH 7.6, 25°C, Gln-dependent ATPase, mutant P690Q Escherichia coli