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Literature summary for 6.3.5.5 extracted from

  • Abdelal, A.T.; Bussey, L.; Vickers, L.
    Carbamoylphosphate synthetase from Pseudomonas aeruginosa. Subunit composition, kinetic analysis and regulation (1983), Eur. J. Biochem., 129, 697-702.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetylornithine activates Pseudomonas aeruginosa
Orn activates Pseudomonas aeruginosa

Inhibitors

Inhibitors Comment Organism Structure
UMP feedback inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
Gln
-
Pseudomonas aeruginosa
17
-
NH4+
-
Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requires free Mg2+, optimal concentration: 4-10 mM Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
12200
-
1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
44000
-
1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
168000
-
sucrose density gradient ultracentrifugation Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
derepressed strain
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.561
-
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ATP + L-Gln + HCO3-
-
Pseudomonas aeruginosa 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
?
2 ATP + NH4+ + HCO3-
-
Pseudomonas aeruginosa 2 ADP + phosphate + carbamoyl phosphate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 44000 + 1 * 12200, SDS-PAGE Pseudomonas aeruginosa
More self-association does not play a role in regulation of enzyme activity Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
triethanolamine buffer Pseudomonas aeruginosa

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 7.5: about 65% of maximal activity, 8.5: about 40% of maximal activity Pseudomonas aeruginosa