Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetylornithine | activates | Pseudomonas aeruginosa | |
Orn | activates | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
UMP | feedback inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
Gln | - |
Pseudomonas aeruginosa | |
17 | - |
NH4+ | - |
Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requires free Mg2+, optimal concentration: 4-10 mM | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
12200 | - |
1 * 44000 + 1 * 12200, SDS-PAGE | Pseudomonas aeruginosa |
44000 | - |
1 * 44000 + 1 * 12200, SDS-PAGE | Pseudomonas aeruginosa |
168000 | - |
sucrose density gradient ultracentrifugation | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
derepressed strain | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.561 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 ATP + L-Gln + HCO3- | - |
Pseudomonas aeruginosa | 2 ADP + phosphate + L-Glu + carbamoyl phosphate | - |
? | |
2 ATP + NH4+ + HCO3- | - |
Pseudomonas aeruginosa | 2 ADP + phosphate + carbamoyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 44000 + 1 * 12200, SDS-PAGE | Pseudomonas aeruginosa |
More | self-association does not play a role in regulation of enzyme activity | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
triethanolamine buffer | Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | 7.5: about 65% of maximal activity, 8.5: about 40% of maximal activity | Pseudomonas aeruginosa |