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Literature summary for 6.3.5.4 extracted from

  • Lea, P.J.; Fowden, L.
    The purification and properties of glutamine-dependent asparagine synthetase isolated from Lupinus luteus (1975), Proc. R. Soc. Lond. B Biol. Sci., 192, 13-26.
No PubMed abstract available

General Stability

General Stability Organism
glycerol and 2-mercaptoethanol are essential for enzyme stability during purification at 4°C Lupinus albus

Inhibitors

Inhibitors Comment Organism Structure
2-Amino-2-carboxy-L-ethanesulfonamide
-
Lupinus albus
2-Hydroxyethyl-L-Gln
-
Lupinus albus
2-oxoglutarate in presence of aminoxyacetate, 50% inhibition at 5 mM Lupinus albus
5-Bromo-4-oxo-L-norvaline
-
Lupinus albus
5-Chloro-4-oxo-L-norvaline
-
Lupinus albus
Albizzine
-
Lupinus albus
AMP 2.5 mM, 50% inhibition Lupinus albus
Asn
-
Lupinus albus
ATP strong inhibition above 5 mM Lupinus albus
azaserine
-
Lupinus albus
beta-Methyl-DL-Asn
-
Lupinus albus
Ca2+
-
Lupinus albus
Cu2+
-
Lupinus albus
diphosphate 5 mM, 45% inhibition Lupinus albus
DL-homo-Gln
-
Lupinus albus
erythro-beta-Hydroxy-L-Asn
-
Lupinus albus
erythro-beta-hydroxy-L-Asp
-
Lupinus albus
erythro-beta-Methyl-L-Asp
-
Lupinus albus
gamma-Methylene-L-Gln
-
Lupinus albus
Gln D-Gln, weak Lupinus albus
Glu 150 mM, 50% inhibition Lupinus albus
L-Glutamate diamide
-
Lupinus albus
L-Glutamate-gamma-ethyl ester
-
Lupinus albus
L-glutamate-gamma-methyl ester
-
Lupinus albus
L-methionine-S-sulfoximine
-
Lupinus albus
Li+
-
Lupinus albus
meso-Diaminosuccinamate
-
Lupinus albus
N-acetyl-L-Gln
-
Lupinus albus
N-alpha-Methyl-L-Asn
-
Lupinus albus
N-Carbobenzoxy-DL-Gln
-
Lupinus albus
N-ethyl-L-Asn
-
Lupinus albus
N-Methyl-L-Asn
-
Lupinus albus
S-Carbamoylcysteine
-
Lupinus albus
Sn2+
-
Lupinus albus
Sr2+
-
Lupinus albus
threo-beta-Hydroxy-L-Asn
-
Lupinus albus
threo-beta-methyl-L-Asp
-
Lupinus albus
Zn2+
-
Lupinus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
L-Gln
-
Lupinus albus
0.15
-
ATP
-
Lupinus albus
0.8
-
L-Asp
-
Lupinus albus
2.1
-
NH4+
-
Lupinus albus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Lupinus albus 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ can partially replace Mg2+ in activation Lupinus albus
Mg2+ Km: 1.1 mM Lupinus albus
Mn2+ can partially substitute for Mg2+ Lupinus albus
Ni2+ can partially replace Mg2+ in activation Lupinus albus

Organism

Organism UniProt Comment Textmining
Lupinus albus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Lupinus albus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.193
-
-
Lupinus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-Asp + L-Gln
-
Lupinus albus AMP + diphosphate + Asn + Glu
-
?
ATP + L-Asp + NH3 30% of the activity relative to Gln Lupinus albus AMP + diphosphate + Asn
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9 8.3
-
Lupinus albus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5
-
AMP
-
Lupinus albus
150
-
Glu
-
Lupinus albus