Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.3.5.3 extracted from

  • Tanwar, A.S.; Goyal, V.D.; Choudhary, D.; Panjikar, S.; Anand, R.
    Importance of hydrophobic cavities in allosteric regulation of formylglycinamide synthetase: insight from xenon trapping and statistical coupling analysis (2013), PLoS ONE, 8, e77781.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.5.3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21DE3-834 (gold) cells Salmonella enterica subsp. enterica serovar Typhimurium

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants R1263A and F209W, vapor diffusion method Salmonella enterica subsp. enterica serovar Typhimurium

Protein Variants

Protein Variants Comment Organism
F209W both the secondary structure content and activity of the mutant enzyme is very similar to the wild type protein Salmonella enterica subsp. enterica serovar Typhimurium
L1181F the mutation causes a 60% loss in activity Salmonella enterica subsp. enterica serovar Typhimurium
L1181W the mutant has secondary structure content and enzyme at par with the wild type protein and exhibits almost no destabilization Salmonella enterica subsp. enterica serovar Typhimurium
L1181Y the mutation results in failure to yield soluble protein Salmonella enterica subsp. enterica serovar Typhimurium
R1263A the mutant is structurally identical to the the native protein Salmonella enterica subsp. enterica serovar Typhimurium
T683W the mutation causes a 70% loss in activity Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O Salmonella enterica subsp. enterica serovar Typhimurium
-
 ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium P74881
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O
-
 Salmonella enterica subsp. enterica serovar Typhimurium ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
FGAM synthetase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
FGAR-AT
-
 Salmonella enterica subsp. enterica serovar Typhimurium
formylglycinamide ribonucleotide amidotransferase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
formylglycinamide synthetase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
PurL
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42 80 the first unfolding transition at 42°C leads to a loss of 15-20% of the secondary structure content of the protein and 80% loss in activity. The second unfolding transition at 80°C represents unfolding of the bulk of the protein Salmonella enterica subsp. enterica serovar Typhimurium

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium