General Stability | Organism |
---|---|
K+ required for stabilization | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-diazo-5-oxo-L-norleucine | - |
Gallus gallus | |
ADP | - |
Gallus gallus | |
albizziin | presence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ inhibits inactivation | Gallus gallus | |
beta-gamma-5'-Adenylyl methylene diphosphate | - |
Gallus gallus | |
Cyanate | - |
Gallus gallus | |
hydroxylamine | - |
Gallus gallus | |
iodoacetamide | rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ | Gallus gallus | |
iodoacetate | rate of inactivation is 20-30times greater in the presence than in the absence of beta-5'-phosphoribosylformylglycinamide, ATP, and Mg2+ | Gallus gallus | |
L-2-Amino-4-oxo-5-chloropentanoic acid | - |
Gallus gallus | |
Na+ | - |
Gallus gallus | |
O-diazoacetyl-L-Ser | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Gallus gallus | |
additional information | - |
additional information | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | can replace Mg2+ in activation | Gallus gallus | |
K+ | - |
Mus musculus | |
K+ | enhances the rate of reaction by 20% up to a concentration of 0.1 M, gradual inhibition at higher concentrations | Gallus gallus | |
Mg2+ | required | Gallus gallus | |
Mn2+ | can replace Mg2+ in activation | Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
133000 | - |
1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization | Gallus gallus |
133000 | 135000 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
133000 | 135000 | - |
Gallus gallus |
133000 | 135000 | - |
Pigeon |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-phosphoribosylformylglycinamide + L-Gln | Gallus gallus | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Mus musculus | - |
- |
- |
Pigeon | - |
- |
- |
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + N2-formyl-N1-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N1-(5-phospho-D-ribosyl)acetamidine + L-glutamate | ping-pong mechanism | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
liver | - |
Gallus gallus | - |
liver | - |
Pigeon | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-phosphoribosylformylglycinamide + L-Gln | - |
Gallus gallus | ? | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Gallus gallus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Mus musculus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + L-Gln + H2O | - |
Pigeon | ADP + phosphate + 5'-phosphoribosylformylglycinamidine + L-Glu | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O | - |
Gallus gallus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine | - |
? | |
ATP + 5'-phosphoribosylformylglycinamide + NH4+ + H2O | - |
Mus musculus | ADP + phosphate + 5'-phosphoribosylformylglycinamidine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 133000, SDS-PAGE. When stored in absence of a reducing agent, e.g. DTT or 2-mercaptoethanol, the enzyme undergoes polymerization | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
highest stability, rather unstable at pH 8 | Gallus gallus |