Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DTT | stimulates | Mus musculus |
General Stability | Organism |
---|---|
thawing and refreezing does not diminish activity | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,N6-Ethenoadenosine 5'-triphosphate | - |
Mus musculus | |
2-mercaptoethanol | - |
Mus musculus | |
6-thioXMP | - |
Mus musculus | |
adenine 9-beta-D-arabinofuranoside 5'-triphosphate | - |
Mus musculus | |
CTP | - |
Mus musculus | |
diphosphate | - |
Mus musculus | |
p-chloromercuribenzoate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
XMP | - |
Mus musculus | |
0.28 | - |
ATP | - |
Mus musculus | |
0.68 | - |
Gln | - |
Mus musculus | |
36 | - |
NH4+ | - |
Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
85000 | - |
gel filtration | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
Ehrlich ascites carcinoma cell | - |
Mus musculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0428 | - |
- |
Mus musculus |
Storage Stability | Organism |
---|---|
-70°C, stable for at least 1 year | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-ribosyl-4,6-dihydroxypyrazolo[3,4-d]-pyrimidine 5-phosphate | - |
Mus musculus | AMP + phosphate + 1-ribosyl-4-hydroxy-6-aminopyrazolo[3,4-d]pyrimidine 5'-phosphate | - |
? | |
ATP + 6-thioXMP + NH4+ | - |
Mus musculus | AMP + phosphate + 6-thioGMP | - |
? | |
ATP + 8-azaXMP + NH4+ | - |
Mus musculus | AMP + phosphate + 8-azaGMP | - |
? | |
ATP + beta-arabinofuranosyl-XMP | - |
Mus musculus | AMP + phosphate + beta-arabinofuranosylGMP | - |
? | |
ATP + dXMP + NH4+ | at 4.3% of the activity relative to XMP | Mus musculus | AMP + phosphate + dGMP | - |
? | |
ATP + XMP + Gln | higher Km value for NH4+ compared with Gln | Mus musculus | AMP + diphosphate + GMP + Glu | - |
? | |
ATP + XMP + NH4+ | higher Km value for NH4+ compared with Gln | Mus musculus | AMP + diphosphate + GMP | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Mus musculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.4 | 8.6 | more than 80% of maximal activity at pH 7.4 and 8.6 | Mus musculus |