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Literature summary for 6.3.5.1 extracted from

  • Resto, M.; Yaffe, J.; Gerratana, B.
    An ancestral glutamine-dependent NAD(+) synthetase revealed by poor kinetic synergism (2009), Biochim. Biophys. Acta, 1794, 1648-1653.
    View publication on PubMed
Search for more literature for 6.3.5.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, with SUMO tag Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
 deamido-NAD+ pH 8.3, 37°C Thermotoga maritima
0.095
-
 ATP pH 8.3, 37°C Thermotoga maritima
0.33
-
 NH3 pH 8.3, 37°C Thermotoga maritima
0.42
-
 L-Gln pH 8.3, 37°C Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + deamido-NAD+ + L-Gln
-
 Thermotoga maritima AMP + diphosphate + NAD+ + L-Glu
-
 ?
ATP + deamido-NAD+ + NH3 + H2O
-
 Thermotoga maritima AMP + diphosphate + NAD+
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 80 at temperatures above 65°C the glutamine-dependent reaction becomes about 20% faster than the ammonia-dependent at 80°C Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.15
-
 ATP pH 8.3, 37°C Thermotoga maritima
0.16
-
 deamido-NAD+ pH 8.3, 37°C Thermotoga maritima
0.24
-
 NH3 pH 8.3, 37°C Thermotoga maritima
0.288
-
 L-Gln pH 8.3, 37°C Thermotoga maritima

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.68
-
 L-Gln pH 8.3, 37°C Thermotoga maritima
0.7
-
 NH3 pH 8.3, 37°C Thermotoga maritima
1.6
-
 ATP pH 8.3, 37°C Thermotoga maritima
7.6
-
 deamido-NAD+ pH 8.3, 37°C Thermotoga maritima