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Literature summary for 6.3.5.1 extracted from
- Heterologous expression, purification, and enzymatic activity of Mycobacterium tuberculosis NAD(+) synthetase (2002), Protein Expr. Purif., 25, 547-557.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| most efficient expression of His-tagged NadE-738 and NadE-679 in Escherichia coli strain Origami | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.52 | - |
deamido-NAD+ | 37°C, pH 8.0, recombinant NadE-679 | Mycobacterium tuberculosis |  |
| 0.79 | - |
ATP | 37°C, pH 8.0, recombinant NadE-679 | Mycobacterium tuberculosis | |
| 1.1 | - |
L-glutamine | 37°C, pH 8.8, recombinant NadE-738 | Mycobacterium tuberculosis | |
| 1.3 | - |
ATP | 37°C, pH 8.5, recombinant NadE-738 | Mycobacterium tuberculosis | |
| 1.6 | - |
L-glutamine | 37°C, pH 8.5, recombinant NadE-679 | Mycobacterium tuberculosis | |
| 2.5 | - |
NH4+ | 37°C, pH 8.0, recombinant NadE-679 | Mycobacterium tuberculosis | |
| 2.9 | - |
deamido-NAD+ | 37°C, pH 8.5, recombinant NadE-738 | Mycobacterium tuberculosis | |
| 27 | - |
NH4+ | 37°C, pH 8.5, recombinant NadE-738 | Mycobacterium tuberculosis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 67000 | - |
8 * 67000, SDS-PAGE | Mycobacterium tuberculosis |
| 600000 | - |
gel filtration, NadE-679 | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deamido-NAD+ + L-glutamine + H2O | Mycobacterium tuberculosis | - |
AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant NadE-738 and NadE-679, Ni2+-nitrilotriacetic acid agarose, gel filtration | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.033 | - |
recombinant NadE-738, nitrogen donor glutamine | Mycobacterium tuberculosis |
| 0.1 | - |
recombinant NadE-738, nitrogen donor NH4+ | Mycobacterium tuberculosis |
| 0.44 | - |
recombinant NadE-679, nitrogen donor glutamine | Mycobacterium tuberculosis |
| 0.67 | - |
recombinant NadE-679, nitrogen donor NH4+ | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deamido-NAD+ + L-glutamine + H2O | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ + L-glutamate | - |
? | |
| ATP + deamido-NAD+ + NH4+ + H2O | - |
Mycobacterium tuberculosis | AMP + diphosphate + NAD+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| octamer | 8 * 67000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NadE-679 | short NadE form, probably raising from translation of NadE gene from an internal start codon | Mycobacterium tuberculosis |
| NadE-679 | - |
Mycobacterium tuberculosis |
| NadE-738 | - |
Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
recombinant NadE-679, nitrogen donor NH4+ | Mycobacterium tuberculosis |
| 8.5 | - |
recombinant NadE-738, nitrogen donor NH4+, recombinant NadE-679, nitrogen donor glutamine | Mycobacterium tuberculosis |
| 8.8 | - |
recombinant NadE-738, nitrogen donor glutamine | Mycobacterium tuberculosis |
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