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Literature summary for 6.3.5.1 extracted from
- Partial purification and properties of nicotinamide adenine dinucleotide synthetase from human erythrocytes: evidence that enzyme activity is a sensitive indicator of lead exposure (1990), Blood, 75, 1576-1582.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | erythrocyte NAD synthetase is activated by lead, and the activity is a sensitive indicator of lead exposure in human | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| EDTA and KCl increase stability substantially | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Pb2+ | - |
Homo sapiens |  |
| Zn2+ | - |
Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.108 | - |
deamido-NAD+ | - |
Homo sapiens | |
| 0.154 | - |
ATP | - |
Homo sapiens | |
| 2.17 | - |
Gln | - |
Homo sapiens | |
| 64.2 | - |
NH4+ | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required | Homo sapiens | |
| K+ | Km: 8.32 mM | Homo sapiens | |
| Mg2+ | required | Homo sapiens | |
| Mg2+ | Km: 1.36 mM | Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 483000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.017 | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 24 h, complete loss of activity | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + deamido-NAD+ + L-Gln | - |
Homo sapiens | AMP + diphosphate + NAD+ + L-Glu | - |
? | |
| ATP + deamido-NAD+ + NH4+ | - |
Homo sapiens | AMP + diphosphate + NAD+ | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.4 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | 6.0: about 75% of maximal activity, 8.0: about 55% of maximal activity | Homo sapiens |
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