Literature summary for 6.3.4.5 extracted from

Brasse-Lagnel, C.; Lavoinne, A.; Fairand, A.; Vavasseur, K.; Deniel, N.; Husson, A.
Biphasic effect of IL-1beta on the activity of argininosuccinate synthetase in Caco-2 cells. Involvement of nitric oxide production (2006), Biochimie, 88, 607-612.

Search for more literature for 6.3.4.5

Activating Compound

Activating Compound	Comment	Organism	Structure
IL-1beta	ASS is rapidly induced by a short time exposure of IL-1beta to Caco-2 cells. By contrast, a long-term 24 h exposure to IL-1beta inhibited the ASS activity despite an increase in both specific mRNA-level and protein amount, demonstrating a post-translational effect	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
IL-1beta	a long-term 24 h exposure of ASS to IL-1beta in Caco-2 cells inhibits its activity. The inhibiting effect of IL-1beta is linked to the production of nitric oxide (NO) induced by IL-1beta. The inhibiting effect is totally blocked in the presence of N-methyl-L-arginine an inhibitor of the inducible nitric oxide synthase or by culturing the cells in an arginine-deprived medium	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
additional information	it is shown that IL-1beta inhibits the ASS activity through a post-translational mechanism since increase in both specific mRNA-level and protein amount can be detected after long-time exposure of Caco-2 cells to IL-1beta. The inhibiting effect of IL-1beta is linked to the production of nitric oxide (NO) induced by IL-1beta	Homo sapiens
nitrosylation	exposure of ASS to IL-1beta in Caco-2 cells induces the nitrosylation of the ASS protein	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
adenocarcinoma cell line	human adenocarcinoma cell line Caco-2/TC7	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1	-	specific activity of ASS in Caco-2 cells after long time exposure (24h) to 1 ng/ml IL-1beta	Homo sapiens
1.27	-	specific activity of ASS in Caco-2 cells after the addition of 0.5 mM S-nitroso-acetylpenicillamine for 24h, an NO donor. This clearly demonstrates that NO induces an inhibition of the ASS activity in Caco-2 cells.	Homo sapiens
2.5	-	specific activity of ASS in Caco-2 cells after short time exposure (4h) to 1 ng/ml IL-1beta	Homo sapiens

Synonyms

Synonyms	Comment	Organism
Argininosuccinate synthetase	-	Homo sapiens
ASS	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)