Literature summary for 6.3.4.4 extracted from

Bouyoub, A.; Barbier, G.; Forterre, P.; Labedan, B.
The adenylosuccinate synthetase from the hyperthermophilic archaeon Pyrococcus species displays unusual structural features (1996), J. Mol. Biol., 261, 144-154.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli. The archaeal adenylosuccinate synthetase has a significant number of substitutions in residues that are conserved in all other homologous enzymes from bacteria to man. Despite theses differences the purA-like archaeal gene seems to be normally expressed and its product functions in vivo in bacteria, since it complements an Escherichia coli purA auxotroph	Pyrococcus sp.
the archaeal adenylosuccinate synthase has a significant number of substitutions in residues that are conserved in all other homologous enzymes from bacteria to human. In Escherichia coli, the conserved residues are essential for catalytic activity and/or for the folded structure of the homodimer. Despite these drastic differences, the purA-like archaeal gene is normally expressed and its product functions in vivo in bacteria, since it complements an Escherichia coli purA auxotroph	Pyrococcus sp.

Cloned (Comment)

Organism

expression in Escherichia coli. The archaeal adenylosuccinate synthetase has a significant number of substitutions in residues that are conserved in all other homologous enzymes from bacteria to man. Despite theses differences the purA-like archaeal gene seems to be normally expressed and its product functions in vivo in bacteria, since it complements an Escherichia coli purA auxotroph

Pyrococcus sp.

the archaeal adenylosuccinate synthase has a significant number of substitutions in residues that are conserved in all other homologous enzymes from bacteria to human. In Escherichia coli, the conserved residues are essential for catalytic activity and/or for the folded structure of the homodimer. Despite these drastic differences, the purA-like archaeal gene is normally expressed and its product functions in vivo in bacteria, since it complements an Escherichia coli purA auxotroph

Pyrococcus sp.

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus sp.	Q59726	-	-
Pyrococcus sp. ST700	Q59726	-	-

Synonyms

Synonyms	Comment	Organism
purA	-	Pyrococcus sp.

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Release 2023.1 (January 2023)