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Literature summary for 6.3.4.4 extracted from

  • Wang, X.; Akasaka, R.; Takemoto, C.; Morita, S.; Yamaguchi, M.; Terada, T.; Shirozu, M.; Yokoyama, S.; Chen, S.; Si, S.; Xie, Y.
    Overexpression, purification, crystallization and preliminary crystallographic studies of a hyperthermophilic adenylosuccinate synthetase from Pyrococcus horikoshii OT3 (2011), Acta Crystallogr. Sect. F, 67, 1551-1555.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting-drop vapour-diffusion method. Crystal belong to the trigonal space group P3(2)12, with unit-cell parameters a = b = 57.2, c = 107.9 A. There is a single molecule in the asymmetric unit Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
1 * 80000, the enzyme mainly exists as dimer in solution, SDS-PAGE Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O58187
-
-
Pyrococcus horikoshii OT-3 O58187
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Subunits

Subunits Comment Organism
dimer 1 * 80000, the enzyme mainly exists as dimer in solution, SDS-PAGE Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
AdSS
-
Pyrococcus horikoshii