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Literature summary for 6.3.4.4 extracted from

  • Kang, C.; Sun, N.; Honzatko, R.B.; Fromm, H.J.
    Replacement of Asp333 with Asn by site-directed mutagenesis changes the substrate specificity of Escherichia coli adenylosuccinate synthetase from guanosine 5'-triphosphate to xanthosine 5'-triphosphate (1994), J. Biol. Chem., 269, 24046-24049.
    View publication on PubMed
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Protein Variants

Protein Variants Comment Organism
D333E mutant enzymes D333N, D333E, and D333Q show decreased turnover numbers and increased Km values for GTP. The three mutants each have higher affinity for XTP and ITP than does the wild-type enzyme Escherichia coli
D333N mutant enzymes D333N, D333E, and D333Q show decreased turnover numbers and increased Km values for GTP. The three mutants each have higher affinity for XTP and ITP than does the wild-type enzyme Escherichia coli
D333Q mutant enzymes D333N, D333E, and D333Q show decreased turnover numbers and increased Km values for GTP. The three mutants each have higher affinity for XTP and ITP than does the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
 GTP wild-type Escherichia coli
0.0286
-
 XTP mutant D333E Escherichia coli
0.0331
-
 XTP mutant D333N Escherichia coli
0.054
-
 IMP XTP, mutant D333Q Escherichia coli
0.14
-
 GTP mutant D333E Escherichia coli
0.193
-
 GTP mutant D333N Escherichia coli
0.334
-
 GTP mutant D33Q Escherichia coli
0.388
-
 XTP wild-type Escherichia coli
1.07
-
 ITP mutant D333Q Escherichia coli
1.27
-
 UTP mutant D333N Escherichia coli
2.06
-
 UTP wild-type Escherichia coli
2.87
-
 ITP mutant D333E Escherichia coli
3.29
-
 ITP mutant D333N Escherichia coli
3.41
-
 UTP mutant D333Q Escherichia coli
4.38
-
 UTP mutant D333E Escherichia coli
17.3
-
 ITP wild-type Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 wild-type and mutant enzymes D333N, D333E, and D333Q
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-Asp
-
 Escherichia coli GDP + phosphate + adenylosuccinate
-
 ?
ITP + IMP + L-Asp
-
 Escherichia coli IDP + phosphate + adenylosuccinate
-
 ?
UTP + IMP + L-Asp
-
 Escherichia coli UDP + phosphate + adenylosuccinate
-
 ?
XTP + IMP + L-Asp
-
 Escherichia coli XDP + phosphate + adenylosuccinate
-
 ?