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Literature summary for 6.3.4.4 extracted from

  • Kang, C.; Sun, N.; Poland, B.W.; Gorrell, A.; Honzatko, R.B.; Fromm, H.J.
    Residues essential for catalysis and stability of the active site of Escherichia coli adenylosuccinate synthetase as revealed by directed mutation and kinetics (1997), J. Biol. Chem., 272, 11881-11885.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
D13A mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme Escherichia coli
E14A mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme Escherichia coli
H41N mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.021
-
IMP wild-type Escherichia coli
0.022
-
GTP wild-type Escherichia coli
0.023
-
GTP GTP, mutant K16Q Escherichia coli
0.031
-
GTP GTP, mutant E14A Escherichia coli
0.035
-
GTP IMP, mutant R131L Escherichia coli
0.035
-
GTP GTP, mutant R131L Escherichia coli
0.04
-
IMP mutant H41N Escherichia coli
0.0505
-
IMP mutant K16Q Escherichia coli
0.055
-
IMP mutant E14A Escherichia coli
0.126
-
GTP mutant H41N Escherichia coli
0.28
-
Asp wild-type Escherichia coli
0.308
-
Asp Asp, mutant K16Q Escherichia coli
1.4
-
Asp mutant E14A Escherichia coli
1.95
-
Asp mutant H41N Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
wild-type and mutant enzymes D13A, E14A, H41N, and K16Q
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + IMP + L-Asp
-
Escherichia coli GDP + phosphate + adenylosuccinate
-
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Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli