Protein Variants | Comment | Organism |
---|---|---|
D13A | mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme | Escherichia coli |
E14A | mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme | Escherichia coli |
H41N | mutant enzyme D13A shows no measurable activity, mutant enzymes E14A and H41N exhibit 1% of the activity of the wild-type enzyme and 2-7fold increases in Km of substrates. The mutant enzyme K16Q has 34% of the activity of wild-type enzyme and Km values for substrates are virtually unchanged from those of the wild-type enzyme | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
IMP | wild-type | Escherichia coli | |
0.022 | - |
GTP | wild-type | Escherichia coli | |
0.023 | - |
GTP | GTP, mutant K16Q | Escherichia coli | |
0.031 | - |
GTP | GTP, mutant E14A | Escherichia coli | |
0.035 | - |
GTP | IMP, mutant R131L | Escherichia coli | |
0.035 | - |
GTP | GTP, mutant R131L | Escherichia coli | |
0.04 | - |
IMP | mutant H41N | Escherichia coli | |
0.0505 | - |
IMP | mutant K16Q | Escherichia coli | |
0.055 | - |
IMP | mutant E14A | Escherichia coli | |
0.126 | - |
GTP | mutant H41N | Escherichia coli | |
0.28 | - |
Asp | wild-type | Escherichia coli | |
0.308 | - |
Asp | Asp, mutant K16Q | Escherichia coli | |
1.4 | - |
Asp | mutant E14A | Escherichia coli | |
1.95 | - |
Asp | mutant H41N | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild-type and mutant enzymes D13A, E14A, H41N, and K16Q | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + IMP + L-Asp | - |
Escherichia coli | GDP + phosphate + adenylosuccinate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Escherichia coli |