KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-phospho-alpha-D-ribose 1-diphosphate | in the coupled reaction, 200fold lower than in the uncoupled reaction | Salmonella enterica subsp. enterica serovar Typhimurium | |
0.33 | - |
ATP | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
1.16 | - |
ITP | good alternative substrate for ATP | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O | the enzyme is a facultative ATPase that uses ATP hydrolysis to drive the synthesis of nicotinate mononucleotide and diphosphate from nicotinic acid and phosphoribosyl diphosphate in a steady-state reaction, the enzyme undergoes phosphorylation in His-219 by bound ATP, phosphorylated enzyme has higher affinity for substrates than does nonphosphorylated enzyme | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate | - |
r | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate | - |
? | |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ITP + H2O | 5-phospho-alpha-D-ribose 1-diphosphate in complex with Mg | Salmonella enterica subsp. enterica serovar Typhimurium | beta-nicotinate D-ribonucleotide + diphosphate + IDP + phosphate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | reaction accelerated 10-30fold | Salmonella enterica subsp. enterica serovar Typhimurium | |
ITP | - |
Salmonella enterica subsp. enterica serovar Typhimurium |