Cloned (Comment) | Organism |
---|---|
overepression in Escherichia coli | Geobacillus kaustophilus |
Crystallization (Comment) | Organism |
---|---|
crystal structure of Geobacillus kaustophilus TilS complexed with Bacillus subtilis tRNAIle CAU at 3.65 A resolution, by the multiwavelength anomalous dispersion method. The asymmetric unit contains one TilS homodimer and two tRNAs, each tightly embedded in one monomer of TilS, with an overall interface of 2998 A. Each monomer consists of an amino-terminal catalytic domain, and two carboxy-terminal domains, connected by a long a-helical linker and a loop linker, respectively | Geobacillus kaustophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00008 | - |
precursor [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus | |
0.00014 | - |
mature [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[tRNAIle2]-cytidine34 + L-lysine + ATP | Geobacillus kaustophilus | - |
[tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus kaustophilus | Q5L3T3 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus kaustophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
mature [tRNAIle2]-cytidine34 + L-lysine + ATP | TilS can modify both types of tRNA, pre-tRNAIle2 and mature tRNAIle2, with comparable efficiencies. TilS specifically recognizes the entire L-shape structure in pre-tRNAIle2 through extensive interactions coupled with sequential domain movements. TilS prevents the recognition of tRNAIle2 by methionyl-tRNA synthetase and achieves high specificity for its substrate | Geobacillus kaustophilus | mature [tRNAIle2]-2-lysylcytidine34 + AMP + diphosphate | - |
? | |
precursor [tRNAIle2]-cytidine34 + L-lysine + ATP | TilS can modify both types of tRNA, pre-tRNAIle2 and mature tRNAIle2, with comparable efficiencies. TilS specifically recognizes the entire L-shape structure in pre-tRNAIle2 through extensive interactions coupled with sequential domain movements. TilS prevents the recognition of tRNAIle2 by methionyl-tRNA synthetase and achieves high specificity for its substrate | Geobacillus kaustophilus | precursor [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? | |
[tRNAIle2]-cytidine34 + L-lysine + ATP | - |
Geobacillus kaustophilus | [tRNAIle2]-2-L-lysylcytidine34 + AMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TilS | - |
Geobacillus kaustophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
precursor [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus | |
0.03 | - |
mature [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
21.4 | - |
mature [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus | |
31.2 | - |
precursor [tRNAIle2]-cytidine34 | pH 7.8, 60°C | Geobacillus kaustophilus |