Literature summary for 6.3.4.15 extracted from

Pendini, N.R.; Yap, M.Y.; Traore, D.A.; Polyak, S.W.; Cowieson, N.P.; Abell, A.; Booker, G.W.; Wallace, J.C.; Wilce, J.A.; Wilce, M.C.
Structural characterization of Staphylococcus aureus biotin protein ligase and interaction partners: an antibiotic target (2013), Protein Sci., 22, 762-773.

Search for more literature for 6.3.4.15

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21 cells	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
apoenzyme, in complex with biotin, and complexed with biotinyl-5'-AMP, hanging drop vapor diffusion method, using	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus ECT-R2	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus ECT-R2	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus ECT-R2	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
additional information	biotin protein ligase is a bifunctional protein, possessing both biotin ligase and transcription repressor activities	Staphylococcus aureus	?	-	?
additional information	biotin protein ligase is a bifunctional protein, possessing both biotin ligase and transcription repressor activities	Staphylococcus aureus ECT-R2	?	-	?

Synonyms

Synonyms	Comment	Organism
biotin protein ligase	-	Staphylococcus aureus
BPL	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Staphylococcus aureus

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Release 2023.1 (January 2023)