Cloned (Comment) | Organism |
---|---|
expressed as thioredoxin- and maltose binding protein-fusion proteins in Escherichia coli BL21(DE3) cells | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli AVB101 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography, Ni-NTA resin column chromatography, and Superdex 75 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + Avi-tagged thioredoxin | biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) | Escherichia coli | ? | - |
? | |
ATP + biotin + Avi-tagged thioredoxin | biotin ligase site-specifically biotinylates a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag) | Escherichia coli AVB101 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * about 35000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
biotin ligase | - |
Escherichia coli |
BirA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Escherichia coli |