Literature summary for 6.3.4.15 extracted from

Soares da Costa, T.P.; Yap, M.Y.; Perugini, M.A.; Wallace, J.C.; Abell, A.D.; Wilce, M.C.; Polyak, S.W.; Booker, G.W.
Dual roles of F123 in protein homodimerization and inhibitor binding to biotin protein ligase from Staphylococcus aureus (2014), Mol. Microbiol., 91, 110-120.

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Protein Variants

Protein Variants	Comment	Organism
F123G	the mutation has minimal effect upon the Km value for MgATP and biotin. In contrast, the kcat for biotin and MgATP are significantly compromised to about 10% that of wild type enzyme	Staphylococcus aureus
F123R	the mutation has minimal effect upon the Km value for MgATP and biotin. In contrast, the kcat for biotin and MgATP are significantly compromised to about 10% that of wild type enzyme	Staphylococcus aureus

Inhibitors

Inhibitors	Comment	Organism	Structure
biotin acetylene	i.e. (3aS,4S,6aR)-4-hept-6-ynyl-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one	Staphylococcus aureus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required for activity	Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38300	-	2 * 38300, calculated from amino acid sequence	Staphylococcus aureus
76000	-	sedimentation equilibrium analysis	Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	Staphylococcus aureus ECT-R2	-	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	-	-	-
Staphylococcus aureus ECT-R2	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA column chromatography, and Superdex 200 gel filtration	Staphylococcus aureus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?
ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	Staphylococcus aureus ECT-R2	AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 38300, calculated from amino acid sequence	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
biotin protein ligase	-	Staphylococcus aureus
BPL	-	Staphylococcus aureus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Staphylococcus aureus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0003	-	biotin acetylene	wild type enzyme, pH and temperature not specified in the publication	Staphylococcus aureus
0.0072	-	biotin acetylene	mutant enzyme F123G, pH and temperature not specified in the publication	Staphylococcus aureus
0.0076	-	biotin acetylene	mutant enzyme F123R, pH and temperature not specified in the publication	Staphylococcus aureus

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Release 2023.1 (January 2023)