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Literature summary for 6.3.4.15 extracted from

  • Daniels, K.; Beckett, D.
    Biochemical properties and biological function of a monofunctional microbial biotin protein ligase (2010), Biochemistry, 49, 5358-5365.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.4.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
 2 * 26000, calculated. Equilibrium analytical ultracentrifugation measurements performed on the apoenzyme and its complexes with biotin and bio-5-AMP all yield molecular weights for the protein consistent with a dimer. Regardless of ligation state the Pyrococcus horikoshii enzyme is a dimer Pyrococcus horikoshii
51200
-
 sedimentation equilibrium study Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii O57883
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Pyrococcus horikoshii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 biotin binding shows an equilibrium constant of 200 nMat 20°C. The equilibrium dissociation constant increases by approximately four fold on increasing the temperature from 10°C to 50°C Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + biotin + apo-[acetyl CoA carboxylase] no coupling is observed in ATP and biotin binding to the BPL Pyrococcus horikoshii AMP + diphosphate + acetyl CoA carboxylase
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 26000, calculated. Equilibrium analytical ultracentrifugation measurements performed on the apoenzyme and its complexes with biotin and bio-5'-AMP all yield molecular weights for the protein consistent with a dimer. Regardless of ligation state the Pyrococcus horikoshii enzyme is a dimer Pyrococcus horikoshii

Synonyms

Synonyms Comment Organism
BPL
-
 Pyrococcus horikoshii