Crystallization (Comment) | Organism |
---|---|
in absence and presence of ATP. Upon ATP binding, the central B-domain closes. Residues G165 and G166 play a role in ATP binding | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G165V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
G165V/G166V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
G166V | the mutation does not affect the maximal velocity of a partial reaction, the bicarbonate-dependent ATPase activity. Km values for ATP increases over 40fold when compared with wild-type. The maximal velocity for the biotin-dependent ATPase activity, i.e. the complete reaction, decreases over 100fold | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
HCO3- | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
0.8 | - |
HCO3- | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
1.7 | - |
HCO3- | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
3.3 | - |
ATP | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
3.4 | - |
ATP | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
3.8 | - |
ATP | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
186 | - |
biotin | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
256 | - |
biotin | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
321 | - |
biotin | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin + HCO3- | - |
Escherichia coli | ADP + phosphate + carboxybiotin | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0016 | - |
HCO3- | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli | |
0.0016 | - |
HCO3- | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
0.0025 | - |
HCO3- | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
0.0037 | - |
biotin | mutant G166V, pH 8.0, 25°C | Escherichia coli | |
0.005 | - |
biotin | mutant G165V, pH 8.0, 25°C | Escherichia coli | |
0.007 | - |
biotin | mutant G165V/G166V, pH 8.0, 25°C | Escherichia coli |