Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli JM109 | Aquifex aeolicus |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method, X-ray analysis | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000 | Aquifex aeolicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Aquifex aeolicus | BC subunit of pyruvate carboxylase, which is involved in gluconeogenesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + biotin-carboxyl-carrier protein + HCO3- | BC subunit of pyruvate carboxylase, BC/active site structure | Aquifex aeolicus | ADP + phosphate + carboxybiotin-carboxyl-carrier protein | - |
? | |
additional information | BC subunit of pyruvate carboxylase, which is involved in gluconeogenesis | Aquifex aeolicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000 | Aquifex aeolicus |
Synonyms | Comment | Organism |
---|---|---|
PC-beta | BC subunit of pyruvate carboxylase | Aquifex aeolicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | structure of the ATP-binding site | Aquifex aeolicus |