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Literature summary for 6.3.3.3 extracted from
- The purification and properties of dethiobiotin synthetase (1970), J. Biol. Chem., 245, 6558-6566.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0013 | - |
7,8-diaminononanoate | - |
Escherichia coli |  |
| 0.005 | - |
ATP | - |
Escherichia coli | |
| 0.6 | - |
Mg2+ | - |
Escherichia coli | |
| 3.4 | - |
CO2 | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 7,8-diaminononanoate + CO2 | - |
Escherichia coli | ADP + phosphate + dethiobiotin | - |
? | |
| ATP + 7,8-diaminononanoate + CO2 | CO2 in form of HCO3- | Escherichia coli | ADP + phosphate + dethiobiotin | - |
? | |
| ATP + diaminobiotin + CO2 | 37% of the activity relative to 7,8-diaminononanoate | Escherichia coli | ADP + phosphate + biotin | - |
? | |
| CTP + 7,8-diaminononanoate + CO2 | - |
Escherichia coli | CDP + phosphate + dethiobiotin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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