Protein Variants | Comment | Organism |
---|---|---|
Y151 | able to use 5-formyltetrahydrohomofolate as a substrate | Mus musculus |
Y152A | increased turnover rate compared to the wild type enzyme | Mus musculus |
Y152F | decreased turnover rate compared to the wild type enzyme | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-formyl,10-methyltetrahydrofolate | weak inhibitor | Mus musculus | |
5-Formyltetrahydrohomofolate | effective inhibitor | Mus musculus | |
N-(4-{[2-(2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl)ethyl](methyl)amino}benzoyl)-L-glutamic acid | at 37°C in 100 mM MES, pH 6.0 | Mus musculus | |
[6R,S]-5-formyl,10-methyltetrahydrofolateGlu3 | - |
Mus musculus | |
[6R,S]-5-formyl,11-methyltetrahydrohomofolate | - |
Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
5-formyltetrahydrofolate | mutant enzyme Y151A, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
0.01 | - |
5-formyltetrahydrofolate | wild type enzyme, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
0.047 | - |
5-formyltetrahydrofolate | mutant enzyme Y152F, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
3 | - |
5-formyltetrahydrofolate | mutant enzyme Y152A, in 100 mM MES, pH 6.0, at 37°C | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | - |
Mus musculus | ADP + 5,10-methenyltetrahydrofolate + phosphate | - |
ir | |
additional information | 5-formyltetrahydrohomofolate, 10-formyltetrahydrohomofolate, and 5-formyl,10-methyltetrahydrofolate are no substrates | Mus musculus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5,10-Methenyltetrahydrofolate synthetase | - |
Mus musculus |
Methenyltetrahydrofolate synthetase | - |
Mus musculus |
MTHFS | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
5-formyltetrahydrofolate | mutant enzyme Y152A, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
0.92 | - |
5-formyltetrahydrofolate | mutant enzyme Y151A, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
3.33 | - |
5-formyltetrahydrofolate | wild type enzyme, in 100 mM MES, pH 6.0, at 37°C | Mus musculus | |
4.3 | - |
5-formyltetrahydrofolate | mutant enzyme Y152F, in 100 mM MES, pH 6.0, at 37°C | Mus musculus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
5-Formyltetrahydrohomofolate | at 37°C in 100 mM MES, pH 6.0 | Mus musculus | |
0.001 | - |
5-formyl,10-methyltetrahydrofolate | at 37°C in 100 mM MES, pH 6.0 | Mus musculus | |
0.006 | - |
[6R,S]-5-formyl,11-methyltetrahydrohomofolate | at 37°C in 100 mM MES, pH 6.0 | Mus musculus | |
0.02 | - |
[6R,S]-5-formyl,10-methyltetrahydrofolateGlu3 | at 37°C in 100 mM MES, pH 6.0 | Mus musculus |