Literature summary for 6.3.3.2 extracted from

Chen, S.; Shin, D.H.; Pufan, R.; Kim, R.; Kim, S.H.
Crystal structure of methenyltetrahydrofolate synthetase from Mycoplasma pneumoniae (GI: 13508087) at 2.2.ANG. resolution (2004), Proteins, 56, 839-843.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)	Mycoplasma pneumoniae

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution	Mycoplasma pneumoniae

Crystallization (Comment)

Organism

purified recombinant His-tagged wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, 30 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 0.1 M NaCl, 1 mM EDTA, 1 mM DTT, at room temperature, 30 mg/ml selenomethionine-labeled enzyme in 0.2 M ammonium sulfate, 0.1 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, at 20°C, flash freezing of all crystals in a solution containing 0.17 M ammonium sulfate, 0.085 M sodium acetate, 21.25% PEG 4000, 15% PEG 400, pH 4.6, X-ray diffraction structure determination and analysis at 2.2 A resolution

Mycoplasma pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 5-formyltetrahydrofolate	Mycoplasma pneumoniae	-	ADP + 5,10-methenyltetrahydrofolate + phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycoplasma pneumoniae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by metal affinity chromatography to homogeneity	Mycoplasma pneumoniae

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate	active site and substrate binding site structure	Mycoplasma pneumoniae	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5-formyltetrahydrofolate	-	Mycoplasma pneumoniae	ADP + 5,10-methenyltetrahydrofolate + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
5,10-Methenyltetrahydrofolate synthetase	-	Mycoplasma pneumoniae
Methenyltetrahydrofolate synthetase	-	Mycoplasma pneumoniae
MTHFS	-	Mycoplasma pneumoniae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mycoplasma pneumoniae