General Stability | Organism |
---|---|
stabilized by non-ionic detergent Tween 20 | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
5-formyltetrahydrofolate | - |
Oryctolagus cuniculus | |
0.3 | - |
ATP | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Oryctolagus cuniculus | 5829 | - |
mitochondrion | small amounts | Oryctolagus cuniculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | as effective as Mg2+ | Oryctolagus cuniculus | |
Co2+ | as effective as Mg2+ | Oryctolagus cuniculus | |
Mg2+ | required in equivalent concentration with ATP, its only role is to form a complex with ATP | Oryctolagus cuniculus | |
Mn2+ | as effective as Mg2+ | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | Oryctolagus cuniculus | it is proposed that methenyltetrahydrofolate synthetase is a salvage enzyme which converts the nonenzymatically formed and nonmetabolically active 5-formyltetrahydropteroyl-(Glu)n back into the one-carbon donor folate pool. It is also possible that methenyltetrahydrofolate synthetase is part of a regulation system in the cell in which its substrate 5-formyl-tetrahydropteroyl-(Glu)n plays a role as a regulator of one-carbon metabolism by inhibiting other folate requiring enzymes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate | random sequential mechanism | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Storage Stability | Organism |
---|---|
-70°C, 0.1% Tween 20, stable for a long time | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-formyltetrahydrofolate | i.e. leucovorin, ir | Oryctolagus cuniculus | ADP + phosphate + 5,10-methylenetetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | ATP in form of MgATP2- | Oryctolagus cuniculus | ADP + phosphate + 5,10-methenyltetrahydrofolate | - |
? | |
ATP + 5-formyltetrahydrofolate | it is proposed that methenyltetrahydrofolate synthetase is a salvage enzyme which converts the nonenzymatically formed and nonmetabolically active 5-formyltetrahydropteroyl-(Glu)n back into the one-carbon donor folate pool. It is also possible that methenyltetrahydrofolate synthetase is part of a regulation system in the cell in which its substrate 5-formyl-tetrahydropteroyl-(Glu)n plays a role as a regulator of one-carbon metabolism by inhibiting other folate requiring enzymes | Oryctolagus cuniculus | ? | - |
? | |
CTP + 5-formyltetrahydrofolate | as effective as ATP | Oryctolagus cuniculus | CDP + phosphate + 5,10-methylenetetrahydrofolate | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Oryctolagus cuniculus |