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Literature summary for 6.3.2.9 extracted from
- Ability of PknA, a mycobacterial eukaryotic-type serine/threonine kinase, to transphosphorylate MurD, a ligase involved in the process of peptidoglycan biosynthesis (2008), Biochem. J., 415, 27-33.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene murD, murD is located in a cluster near the other cell division genes such as ftsW, co-expression of GST-tagged MurD with MBP-fusion PknA, a mycobacterial eukaryotic-type serine/threonine kinase, in Escherichia coli BL21(DE3) cells leading to phosphorylation of mMurD. Also overexpression of murD in Mycobacterium smegmatis as His-tagged protein yields a phosphorylated enzyme | Mycobacterium tuberculosis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Mycobacterium tuberculosis | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mycobacterium tuberculosis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | Mycobacterium tuberculosis | the ligase MurD is involved in the process of peptidoglycan biosynthesis by catalyzing the addition of D-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | Mycobacterium tuberculosis H37Ra / ATCC 25177 | the ligase MurD is involved in the process of peptidoglycan biosynthesis by catalyzing the addition of D-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | A5U4I2 | gene m-murD or Rv2155c | - |
| Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U4I2 | gene m-murD or Rv2155c | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the mycobacterial eukaryotic-type serine/threonine kinase PknA transphosphorylates MurD | Mycobacterium tuberculosis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant recombinant GST-tagged MurD from Escherichia coli by glutathione affinity chromatography, His-tagged MurD from Mycobacterium smegmatis by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | - |
Mycobacterium tuberculosis | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | the ligase MurD is involved in the process of peptidoglycan biosynthesis by catalyzing the addition of D-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine | Mycobacterium tuberculosis | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | - |
Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
| ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate | the ligase MurD is involved in the process of peptidoglycan biosynthesis by catalyzing the addition of D-glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MurD | - |
Mycobacterium tuberculosis |
| UDP-N-acetylmuramoyl-L-alanine:D-glutamateligase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycobacterium tuberculosis | |
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