Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in insect HighFive cells | Trichoplusia ni |
Crystallization (Comment) | Organism |
---|---|
purified enzyme in 150 mM NaCl, 1 mM DTT, 10 mM HEPES-KOH, pH 7.5, is mixed with 50 mM Tris pH 8.0, 2M ammonium sulfate and 1% PEG400, X-ray diffraction structure determination and analysis at 2.8 A resolution, modeling | Trichoplusia ni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 50000, SDS-PAGE | Trichoplusia ni |
360000 | - |
gel filtration | Trichoplusia ni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | Trichoplusia ni | - |
ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoplusia ni | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from insect HighFive cells by nickel affinity and anion exchange chromatography, followed by gel filtration | Trichoplusia ni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate | - |
Trichoplusia ni | ADP + phosphate + (S)-2-[5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido]succinate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | structure modeling, overview | Trichoplusia ni |
oligomer | x * 50000, SDS-PAGE | Trichoplusia ni |
Synonyms | Comment | Organism |
---|---|---|
4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase | - |
Trichoplusia ni |
PAICS | - |
Trichoplusia ni |
SAICAR synthase | - |
Trichoplusia ni |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Trichoplusia ni |
General Information | Comment | Organism |
---|---|---|
additional information | bifunctional enzyme complex, termed PAICS, harboring 5-aminoimidazole ribonucleotide carboxylase and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase activities, the SAICAR active sites is located in the N-terminal domain of PAICS, structure modeling, overview. In addition to the basic loop responsible for phosphate-binding, the adenine-ribose moiety of the nucleotide sits in a largely hydrophobic pocket sandwiched between beta1-strands of the SAICAR domain | Trichoplusia ni |