Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Homo sapiens | |
ATP | - |
Homo sapiens | |
CMP | - |
Homo sapiens | |
CTP | - |
Homo sapiens | |
GTP | - |
Homo sapiens | |
UTP | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
(R)-4'-phosphopantothenate | with ATP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens | |
0.014 | - |
L-cysteine | with ATP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens | |
0.016 | - |
L-cysteine | with CTP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens | |
0.057 | - |
(R)-4'-phosphopantothenate | with CTP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens | |
0.265 | - |
CTP | at pH 7.6 and 37°C | Homo sapiens | |
0.269 | - |
ATP | at pH 7.6 and 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | activity is supported by Mg2+ | Homo sapiens | |
Mn2+ | activity is supported by Mn2+ | Homo sapiens | |
additional information | not influenced by Zn2+, Fe2+, Ca2+, and Cd2+ | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34940 | - |
- |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4'-phosphopantothenate + L-cysteine | Homo sapiens | - |
AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography, and Mono Q column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + (R)-4'-phosphopantothenate + L-cysteine | - |
Homo sapiens | AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
? | |
CTP + (R)-4'-phosphopantothenate + L-cysteine | - |
Homo sapiens | CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine | - |
? | |
additional information | neither GTP nor UTP support enzyme activity with the human enzyme | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 34940, electrospray mass spectrometry | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CoaB | - |
Homo sapiens |
phosphopantothenoylcysteine synthetase | - |
Homo sapiens |
PPCS | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
(R)-4'-phosphopantothenate | with CTP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens | |
0.56 | - |
(R)-4'-phosphopantothenate | with ATP as cosubstrate, at pH 7.6 and 37°C | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.074 | - |
CTP | at pH 7.6 and 37°C | Homo sapiens | |
0.372 | - |
GTP | at pH 7.6 and 37°C | Homo sapiens | |
0.465 | - |
ATP | at pH 7.6 and 37°C | Homo sapiens | |
0.662 | - |
UTP | at pH 7.6 and 37°C | Homo sapiens | |
3.7 | - |
AMP | at pH 7.6 and 37°C | Homo sapiens | |
4.69 | - |
CMP | at pH 7.6 and 37°C | Homo sapiens |