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Literature summary for 6.3.2.51 extracted from

  • Yao, J.; Patrone, J.D.; Dotson, G.D.
    Characterization and kinetics of phosphopantothenoylcysteine synthetase from Enterococcus faecalis (2009), Biochemistry, 48, 2799-2806.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.3.2.51

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 AI cells Enterococcus faecalis

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
 Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
 (R)-4'-phosphopantothenate at pH 7.6 and 37°C Enterococcus faecalis
0.086
-
 L-cysteine at pH 7.6 and 37°C Enterococcus faecalis
0.156
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 2 * 28000, SDS-PAGE Enterococcus faecalis
28415
-
-
 Enterococcus faecalis
60000
-
 gel filtration Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine Enterococcus faecalis
-
 AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-4'-phosphopantothenate + L-cysteine
-
 Enterococcus faecalis AMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?
CTP + (R)-4'-phosphopantothenate + L-cysteine
-
 Enterococcus faecalis CMP + diphosphate + N-[(R)-4'-phosphopantothenoyl]-L-cysteine
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 28000, SDS-PAGE Enterococcus faecalis
homodimer 2 * 28415, electrospray ionization mass spectroemtry Enterococcus faecalis
homodimer 2 * 28525, calculated from amino acid sequence Enterococcus faecalis

Synonyms

Synonyms Comment Organism
CoaB
-
 Enterococcus faecalis
phosphopantothenoylcysteine synthetase
-
 Enterococcus faecalis
PPCS
-
 Enterococcus faecalis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.9
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis
2.9
-
 L-cysteine at pH 7.6 and 37°C Enterococcus faecalis
2.9
-
 (R)-4'-phosphopantothenate at pH 7.6 and 37°C Enterococcus faecalis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.652
-
 CTP at pH 7.6 and 37°C Enterococcus faecalis