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Literature summary for 6.3.2.4 extracted from

  • Noda, M.; Kawahara, Y.; Ichikawa, A.; Matoba, Y.; Matsuo, H.; Lee, D.G.; Kumagai, T.; Sugiyama, M.
    Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae. Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine (2004), J. Biol. Chem., 279, 46143-46152.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 Streptomyces lavendulae

Inhibitors

Inhibitors Comment Organism Structure
D-alanyl-D-alanine
-
Streptomyces lavendulae
D-cycloserine
-
Streptomyces lavendulae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
ATP
-
Streptomyces lavendulae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-alanine Streptomyces lavendulae
-
ADP + phosphate + D-alanyl-D-alanine
-
?
ATP + D-alanine Streptomyces lavendulae ATCC 25233
-
ADP + phosphate + D-alanyl-D-alanine
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces lavendulae
-
strain ATCC25233
-
Streptomyces lavendulae ATCC 25233
-
strain ATCC25233
-

Purification (Commentary)

Purification (Comment) Organism
-
Streptomyces lavendulae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-alanine
-
Streptomyces lavendulae ADP + phosphate + D-alanyl-D-alanine
-
?
ATP + D-alanine
-
Streptomyces lavendulae ATCC 25233 ADP + phosphate + D-alanyl-D-alanine
-
?
D-cycloserine
-
Streptomyces lavendulae ?
-
?
D-cycloserine
-
Streptomyces lavendulae ATCC 25233 ?
-
?

Synonyms

Synonyms Comment Organism
Ddl
-
Streptomyces lavendulae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.67
-
D-alanine
-
Streptomyces lavendulae

pH Range

pH Minimum pH Maximum Comment Organism
6 9.2 higher DDL activity at pH 9.2 than at pH 6.0-7.5 Streptomyces lavendulae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.06
-
D-alanyl-D-alanine
-
Streptomyces lavendulae
0.92
-
D-cycloserine
-
Streptomyces lavendulae