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Literature summary for 6.3.2.4 extracted from

  • Healy, V.L.; Park, I.S.; Walsh, C.T.
    Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteristic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases (1998), Chem. Biol., 5, 197-207.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
L282R 11fold decrease in kcat for D-alanine, 18fold increase in Km for D-alanine, 23fold decrease in Km for D-serine, 9fold drop in kcat for D-serine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13
-
 D-alanine
-
 Escherichia coli
56
-
 D-serine
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + D-alanine Escherichia coli
-
 ADP + phosphate + D-alanyl-D-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Ddl Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-alanine
-
 Escherichia coli ADP + phosphate + D-alanyl-D-alanine
-
 ?
ATP + D-serine
-
 Escherichia coli ADP + phosphate + D-alanyl-D-serine
-
 ?

Synonyms

Synonyms Comment Organism
Ddl
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6
-
 D-serine
-
 Escherichia coli
31.2
-
 D-alanine
-
 Escherichia coli