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Literature summary for 6.3.2.4 extracted from
- Purification and characterization of the VanB ligase associated with type B vancomycin resistance in Enterococcus faecalis V583 (1994), FEBS Lett., 354, 140-142.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Van B ligase is overproduced in Escherichia coli HB101 harboring recombinant plasmid pAT204 | Enterococcus faecalis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
D-Ala | D-Ala, N-terminal Ala, + D-Ala, VanB | Enterococcus faecalis |  |
| 3 | - |
2-hydroxybutanoate | - |
Enterococcus faecalis | |
| 8.3 | - |
2-hydroxypentanoate | - |
Enterococcus faecalis | |
| 11.4 | - |
D-lactate | - |
Enterococcus faecalis | |
| 34 | - |
D-Ala | C-terminal Ala, reaction with D-Ala + ATP, VanB | Enterococcus faecalis | |
| 69 | - |
ATP | VanB | Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
x * 38000, SDS-PAGE | Enterococcus faecalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacteria | - |
- |
- |
| Enterococcus faecalis | - |
V583, VanB ligase with type B vancomycin resistance | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-Ala + 2-hydroxybutanoate | - |
Enterococcus faecalis | ADP + phosphate + D-Ala-D-2-hydroxybutanoate | - |
? | |
| ATP + D-Ala + 2-hydroxypentanoate | - |
Enterococcus faecalis | ADP + phosphate + D-Ala-D-2-hydroxypentanoate | - |
? | |
| ATP + D-Ala + D-Ala | - |
Bacteria | ADP + phosphate + D-Ala-D-Ala | - |
? | |
| ATP + D-Ala + D-Ala | - |
Enterococcus faecalis | ADP + phosphate + D-Ala-D-Ala | - |
? | |
| ATP + D-Ala + D-lactate | - |
Enterococcus faecalis | ADP + phosphate + D-Ala-D-lactate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 38000, SDS-PAGE | Enterococcus faecalis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Enterococcus faecalis |
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