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Literature summary for 6.3.2.4 extracted from
- Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes (1991), Biochemistry, 30, 1673-1682.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Aminoalkylphosphinate | - |
Escherichia coli | |
| D-Ala-D-Ala | - |
Escherichia coli |  |
| D-cycloserine | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
D-Ala | ATP, ligase A | Escherichia coli | |
| 40 | - |
ATP | ligase B | Escherichia coli | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
2 enzyme forms: ligase A and ligase B | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 12.3 | - |
ligase A | Escherichia coli |
| 31 | - |
ligase B | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + D-Ala + D-Ala | r | Escherichia coli | ADP + phosphate + D-Ala-D-Ala | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.4 | - |
D-Ala | ligase A | Escherichia coli | |
| 17 | - |
D-Ala | ligase B | Escherichia coli | |
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