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Literature summary for 6.3.2.4 extracted from

  • Bugg, T.D.H.; Dutka-Malen, S.; Athur, M.; Courvalin, P.; Walsh, C.T.
    Identification of vancomycin resistance protein VanA as a D-alanine:D-alanine ligase of altered substrate specificity (1991), Biochemistry, 30, 2017-2021.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
D-cycloserine
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Ala + Ala Escherichia coli a possible cellular role of VanA is to synthesize a modified cell-wall component, with D-Ala-D-Met or D-Ala-Phe instead of D-Ala-D-Ala, which is subsequently not recognized by vancomycin ?
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
containing recombinant plasmid pAT214, which expressed the VanA protein
-
Escherichia coli JM105
-
containing recombinant plasmid pAT214, which expressed the VanA protein
-

Purification (Commentary)

Purification (Comment) Organism
Van A Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.9
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Ala + Ala a possible cellular role of VanA is to synthesize a modified cell-wall component, with D-Ala-D-Met or D-Ala-Phe instead of D-Ala-D-Ala, which is subsequently not recognized by vancomycin Escherichia coli ?
-
?
ATP + D-Ala + D-2-aminopentanoate
-
Escherichia coli ADP + phosphate + D-Ala-D-2-aminopentanoate
-
?
ATP + D-Ala + D-2-aminopentanoate
-
Escherichia coli JM105 ADP + phosphate + D-Ala-D-2-aminopentanoate
-
?
ATP + D-Ala + D-Ala
-
Escherichia coli ADP + phosphate + D-Ala-D-Ala
-
?
ATP + D-Ala + D-Ala
-
Escherichia coli JM105 ADP + phosphate + D-Ala-D-Ala
-
?
ATP + D-Ala + D-Met
-
Escherichia coli ADP + phosphate + D-Ala-D-Met
-
?
ATP + D-Ala + D-Met
-
Escherichia coli JM105 ADP + phosphate + D-Ala-D-Met
-
?
ATP + D-Ala + D-norleucine
-
Escherichia coli ADP + phosphate + D-Ala-D-norleucine
-
?
ATP + D-Ala + D-norleucine
-
Escherichia coli JM105 ADP + phosphate + D-Ala-D-norleucine
-
?
ATP + D-Ala + D-Phe
-
Escherichia coli ADP + phosphate + D-Ala-D-Phe
-
?
ATP + D-Ala + D-Phe
-
Escherichia coli JM105 ADP + phosphate + D-Ala-D-Phe
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.92
-
D-Ala
-
Escherichia coli